Engineering Natural Product Biosynthesis
نویسندگان
چکیده
This volume highlights natural products, molecular methods for identifying, and current trends in designing non-natural products.
منابع مشابه
Metabolic engineering for plant natural product biosynthesis in microbes.
Plant natural products (NPs) not only serve many functions in an organism's survivability but also demonstrate important pharmacological activities. Isolation of NPs from native sources is frequently limited by low abundance and environmental, seasonal, and regional variation while total chemical synthesis of what are often complex structures is typically commercially infeasible. Reconstruction...
متن کاملHeterologous biosynthesis of natural product naringenin by co-culture engineering
Co-culture engineering is an emerging approach for microbial biosynthesis of a variety of biochemicals. In this study, E. coli-E. coli co-cultures were developed for heterologous biosynthesis of the natural product naringenin. The co-cultures were composed of two independent E. coli strains dedicated to functional expression of different portions of the biosynthetic pathway, respectively. The c...
متن کاملImproving NADPH availability for natural product biosynthesis in Escherichia coli by metabolic engineering.
With microbial production becoming the primary choice for natural product synthesis, increasing precursor and cofactor availability has become a chief hurdle for the generation of efficient production platforms. As such, we employed a stoichiometric-based model to identify combinations of gene knockouts for improving NADPH availability in Escherichia coli. Specifically, two different model obje...
متن کاملBiosynthesis of the Enterotoxic Pyrrolobenzodiazepine Natural Product Tilivalline
The nonribosomal enterotoxin tilivalline was the first naturally occurring pyrrolobenzodiazepine to be linked to disease in the human intestine. Since the producing organism Klebsiella oxytoca is part of the intestinal microbiota and the pyrrolobenzodiazepine causes the pathogenesis of colitis it is important to understand the biosynthesis and regulation of tilivalline activity. Here we report ...
متن کاملTemporal dynamics of natural product biosynthesis in marine cyanobacteria.
Sessile marine organisms are prolific sources of biologically active natural products. However, these compounds are often found in highly variable amounts, with the abiotic and biotic factors governing their production remaining poorly understood. We present an approach that permits monitoring of in vivo natural product production and turnover using mass spectrometry and stable isotope ((15)N) ...
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ژورنال
عنوان ژورنال: Methods in molecular biology
سال: 2022
ISSN: ['1940-6029', '1064-3745']
DOI: https://doi.org/10.1007/978-1-0716-2273-5